Premium
Proteomic analysis of cytokine induced proteins in human intestinal epithelial cells: Implications for inflammatory bowel diseases
Author(s) -
BarcelóBatllori Sílvia,
André Muriel,
Servis Catherine,
Lévy Nicole,
Takikawa Osamu,
Michetti Pierre,
Reymond Marc,
FelleyBosco Emanuela
Publication year - 2002
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/1615-9861(200205)2:5<551::aid-prot551>3.0.co;2-o
Subject(s) - cytokine , proteomics , inflammatory bowel diseases , inflammatory bowel disease , biology , immunology , medicine , biochemistry , pathology , gene , disease
A role for cytokine regulated proteins in epithelial cells has been suggested in the pathogenesis of inflammatory bowel diseases (IBD). The aim of this study was to identify such cytokine regulated targets using a proteomic functional approach. Protein patterns from 35 S‐radiolabeled homogenates of cultured colon epithelial cells were compared before and after exposure to interferon‐γ, interleukin‐1β and interleukin‐6. Proteins were separated by two‐dimensional polyacrylamide gel electrophoresis. Both autoradiographies and silver stained gels were analyzed. Proteins showing differential expression were identified by tryptic in‐gel digestion and mass spectrometry. Metabolism related proteins were also investigated by Western blot analysis. Tryptophanyl‐tRNA synthetase, indoleamine‐2,3‐dioxygenase, heterogeneous nuclear ribonucleoprotein JKTBP, interferon‐induced 35kDa protein, proteasome subunit LMP2 and arginosuccinate synthetase were identified as cytokine modulated proteins in vitro . Using purified epithelial cells from patients, overexpression of indoleamine‐2,3‐dioxygenase, an enzyme involved in tryptophan metabolism, was confirmed in Crohn’s disease as well as in ulcerative colitis, as compared to normal mucosa. No such difference was found in diverticulitis. Potentially, this observation opens new avenues in the treatment of IBD.