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Rapid two‐dimensional analysis of proteins by ultra‐thin layer gel electrophoresis
Author(s) -
Guttman András,
Csapo Zsolt,
Robbins Dave
Publication year - 2002
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/1615-9861(200204)2:4<469::aid-prot469>3.0.co;2-v
Subject(s) - proteome , capillary electrophoresis , proteomics , chromatography , gel electrophoresis , electrophoresis , automation , laser induced fluorescence , fluorescence , computer science , chemistry , nanotechnology , materials science , bioinformatics , biology , biochemistry , optics , physics , engineering , mechanical engineering , gene
Identification of qualitative and/or quantitative protein expression differences as well as characterization of specific cell proteomes would further advance molecular cell biology research. Today, one of the most commonly used tools for proteome analysis is two‐dimensional gel electrophoresis. Although this technology is informative, it is extremely cumbersome, time‐consuming and lacks automation and proper reproducibility. In this paper, we propose an automated separation/detection system capable of rapid two‐dimensional analysis of proteins by ultra‐thin layer gel electrophoresis with real time imaging of the separated components, using fiber optics based laser induced fluorescence technology. The approach is based on electric field mediated separation in capillary dimensions, along with noncovalent, “ in migratio ” fluorescent staining methodology. The advantage of the technology discussed over existing techniques is its simplicity, speed and good detection sensitivity.