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Detecting single molecules launched from liquid surfaces in a time‐of‐flight mass spectrometer using ultraviolet and infrared lasers
Author(s) -
Twerenbold Damian,
Netuschill Alexandre,
de Rooij Nico,
Luginbuhl Phillipe,
Gerber Daniel,
Gritti Dominique,
Gonin Yuan,
Rossel Frédéric,
Vuilleumier JeanLuc
Publication year - 2002
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/1615-9861(200204)2:4<436::aid-prot436>3.0.co;2-i
Subject(s) - ultraviolet , laser , spectrometer , mass spectrometry , infrared , materials science , far infrared laser , molecule , analytical chemistry (journal) , silicon , optics , optoelectronics , chemistry , chromatography , physics , organic chemistry
The launch of molecules from liquid surfaces in a time of flight mass spectrometer has been investigated using different sample preparation techniques, and by exposing the liquid samples to two different laser wavelengths, 337 nm from a N 2 ultraviolet laser and 10.6 νm from a CO 2 infrared laser. The molecules were detected with cryodetectors measuring the energy of the individual molecules. We present insulin and lysozyme results from samples introduced into the vacuum through a micromachined silicon injector, and from samples consisting of a glycerol droplet deposited directly on the sample holder at the high voltage stage of the ion optics.