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Cell‐free protein synthesis on a microchip
Author(s) -
Tabuchi Mari,
Hino Mami,
Shinohara Yasuo,
Baba Yoshinobu
Publication year - 2002
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/1615-9861(200204)2:4<430::aid-prot430>3.0.co;2-5
Subject(s) - cell free protein synthesis , protein biosynthesis , proteome , protein expression , translation (biology) , t7 rna polymerase , expression vector , cell free system , microbiology and biotechnology , gene expression , in vitro , chemistry , biology , messenger rna , biochemistry , recombinant dna , gene , escherichia coli , bacteriophage
Abstract We evaluated the expression of various proteins by using a cell‐free protein synthesis system with a rapid translation system, a microtube, and a microchip technique. Protein expression was successfully achieved with a microfabricated reaction chamber on a plastic chip. Proteins were expressed effectively by use of expression vectors for T7 RNA polymerase (pET) instead of a plasmid for in vitro expression vector, which is recommended by the manufacture of the rapid translation system. Expression of the proteins depended on the type of proteins chosen. Two mammalian proteins were synthesized simultaneously with two expression vectors of pET species. Effective application of the cell‐free protein synthesis system will enable miniaturization of protein synthesis and mediation between the transcriptome and proteome.