Premium
Improved silver staining protocols for high sensitivity protein identification using matrix‐assisted laser desorption/ionization‐time of flight analysis
Author(s) -
Mortz Ejvind,
Krogh Thomas N.,
Vorum Henrik,
Görg Angelika
Publication year - 2001
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/1615-9861(200111)1:11<1359::aid-prot1359>3.0.co;2-q
Subject(s) - silver stain , staining , mass spectrometry , proteomics , chromatography , matrix assisted laser desorption/ionization , chemistry , desorption , microbiology and biotechnology , biochemistry , biology , genetics , organic chemistry , adsorption , gene
In proteomics it is essential to be able to detect proteins separated by gel electrophoresis at high sensitivity. Silver staining is currently the most popular method. Here we present silver staining protocols that are optimized for staining sensitivity, peptide recovery and compatibility with digestion and mass spectrometry.