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A comparison between Sypro Ruby and ruthenium II tris (bathophenanthroline disulfonate) as fluorescent stains for protein detection in gels
Author(s) -
Rabilloud Thierry,
Strub JeanMarc,
Luche Sylvie,
Dorsselaer Alain van,
Lunardi Joël
Publication year - 2001
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/1615-9861(200104)1:5<699::aid-prot699>3.0.co;2-c
Subject(s) - ruthenium , chemistry , tris , fluorescence , mass spectrometry , chelation , chromatography , staining , inorganic chemistry , biochemistry , medicine , physics , quantum mechanics , pathology , catalysis
A comparison between two fluorescent metal chelates for staining proteins separated by electrophoresis has been carried out. One of these chelates is ruthenium II tris (bathophenanthroline disulfonate) and the other is commercial Sypro Ruby. Both can be efficiently detected either with UV tables or with commercial laser fluorescence scanners. The sensitivity and homogeneity of the stains and the interference with mass spectrometry analysis have been investigated. It appears that both stains perform similarly for protein detection, while ruthenium II tris (bathophenanthroline disulfonate) performs better for mass spectrometry analyses and as cost‐effectiveness ratio. However, Sypro Ruby is easier to use as a stain.