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Identification of protein‐bound carbohydrates by mass spectrometry
Author(s) -
Harvey David J.
Publication year - 2001
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/1615-9861(200102)1:2<311::aid-prot311>3.0.co;2-j
Subject(s) - mass spectrometry , chemistry , chromatography , glycan , fragmentation (computing) , protein mass spectrometry , electrospray ionization , context (archaeology) , capillary electrophoresis–mass spectrometry , top down proteomics , proteomics , identification (biology) , sample preparation in mass spectrometry , biochemistry , computer science , biology , glycoprotein , paleontology , botany , gene , operating system
Abstract This review outlines the use of modern methods of mass spectrometry, mainly based on electrospray ionization and matrix‐assisted lased desorption/ionization, for the identification of protein‐bound carbohydrates. The techniques are briefly described together with methods for glycan release and purification prior to mass spectrometry. Fragmentation of glycans, produced under various conditions, is described in the context of obtaining structural information at the sensitivity required for proteomic work.