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Single molecule detector for mass spectrometry with mass independent detection efficiency
Author(s) -
Twerenbold Damian,
Gerber Daniel,
Gritti Dominique,
Gonin Yvan,
Netuschill Alexandre,
Rossel Frédéric,
Schenker Dominique,
Vuilleumier JeanLuc
Publication year - 2001
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/1615-9861(200101)1:1<66::aid-prot66>3.0.co;2-s
Subject(s) - mass spectrometry , detector , chemistry , ionizing radiation , molecule , analytical chemistry (journal) , spectrometer , hybrid mass spectrometer , selected reaction monitoring , tandem mass spectrometry , chromatography , physics , nuclear physics , irradiation , optics , organic chemistry
Experimental results from equimolar PEG and protein standards samples are presented from a MALDI‐TOF mass spectrometer equipped with both ionizing detectors and the novel single molecule sensitive cryodetectors. The data are consistent with a model hypothesis suggesting that the observed decrease in signal strength in conventional ionizing detector MALDI‐TOF mass spectrometers can be explained by the exponentially decreasing quantum efficiency of ionizing detectors. Cryodetectors, in contrast, have a mass independent detection efficiency of 100% on impact and provide additional information on the molecule state owing to the calorimetric nature of the detection mechanism.