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Toward sol‐gel electrochromatographic separations on a chip
Author(s) -
Morishima Keisuke,
Bennett Bryson D.,
Dulay Maria T.,
Quirino Joselito P.,
Zare Richard N.
Publication year - 2002
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/1615-9314(20021101)25:15/17<1226::aid-jssc1226>3.0.co;2-g
Subject(s) - monolith , photoinitiator , borosilicate glass , masking (illustration) , chromatography , lithography , materials science , electrochromatography , photolithography , resolution (logic) , chemistry , nanotechnology , polymer , optoelectronics , capillary electrophoresis , catalysis , composite material , capillary electrochromatography , computer science , art , biochemistry , visual arts , monomer , artificial intelligence
A porous photopolymerized sol‐gel (PSG) monolith was synthesized in the separation channel of a borosilicate glass chip via UV irradiation (5 min) of a mixture of 3‐methacryloxypropyltrimethoxysilane, an acid catalyst, a porogen, and a photoinitiator. The PSG monolith adhered strongly to the chemically untreated channel walls. The chip was fabricated using standard lithography procedures to give channels that are 35‐μm deep and 90‐μm wide. Masking the other channels defined the 4.7‐cm PSG section in the separation channel. Two dyes Coumarin 314 and 510 were successfully separated within baseline resolution in 225 s when fluorescent detection occurred immediately after the PSG section. The separation time was reduced to 80 s with little loss in resolution by detecting the dyes 1.2 cm from the front of the PSG monolith.