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Peptide sequence determination of peptidosteroids on a single bead by electrospray ionization–mass spectroscopy
Author(s) -
Vanhoenacker Gerd,
Liu Li,
Lynen Frederic,
Madder Annemieke,
De Clercq Pierre,
Sandra Pat
Publication year - 2002
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/1615-9314(20020701)25:10/11<671::aid-jssc671>3.0.co;2-t
Subject(s) - electrospray ionization , chemistry , tripeptide , mass spectrometry , chromatography , peptide , electrospray , sequence (biology) , analytical chemistry (journal) , spectroscopy , biochemistry , physics , quantum mechanics
An electrospray ionization mass spectroscopy (ESI‐MS n ) method has been developed to simultaneously determine the sequence of two strands of tripeptides attached to a steroid‐like scaffold. The peptidosteroid compounds were synthesized by solid‐phase chemistry on TentaGel beads. The sequence of both peptide strands of a compound originating from a single bead could be determined. High‐performance liquid chromatography hyphenated to MS n was also applied to a mixture of two isomeric peptidosteroids for sequence determination of their peptide chains.