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Detection of the radical and reduced forms of α‐(4‐pyridyl 1‐oxide)‐ N‐tert ‐butylnitrone/pentyl radical adduct formed on reaction of an oxidized linoleic acid with ferrous ions using high performance liquid chromatography with electrochemical detection
Author(s) -
Iwahashi Hideo,
Nishizaki Koji,
Takagi Ichiro
Publication year - 2002
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/1615-9314(20020601)25:9<601::aid-jssc601>3.0.co;2-i
Subject(s) - chemistry , adduct , ferrous , high performance liquid chromatography , spin trapping , hydroxyl radical , linoleic acid , chlorogenic acid , radical ion , radical , elution , photochemistry , chromatography , medicinal chemistry , organic chemistry , ion , fatty acid
Pentyl radical was detected in the reaction mixture containing an oxidized linoleic acid using high performance liquid chromatography with electrochemical detection (HPLC‐ED) in combination with spin trapping with α‐(4‐pyridyl 1‐oxide)‐ N‐tert ‐butylnitrone (4‐POBN). Both reduced and radical forms of 4‐POBN/pentyl radical adduct were detected in the elution profile of the HPLC‐ED set‐up. Concentrations of the radical and reduced forms of 4‐POBN/pentyl radical adduct which form in the reaction mixture of an oxidized linoleic acid with ferrous ion were 590 nmol/L and 11 nmol/L, respectively. Chlorogenic acid, a polyphenol, inhibited the formation of both reduced and radical forms of 4‐POBN/pentyl radical adduct.