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Thermodynamic behaviour in capillary electrochromatography
Author(s) -
Jiskra Jan,
Claessens Henk A.,
Cramers Carel A.
Publication year - 2002
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/1615-9314(20020601)25:9<569::aid-jssc569>3.0.co;2-3
Subject(s) - capillary electrochromatography , chemistry , electrochromatography , gibbs free energy , enthalpy , chromatography , capillary action , high performance liquid chromatography , entropy (arrow of time) , thermodynamics , capillary electrophoresis , physics
The thermodynamic behaviour of solutes was examined on four reversed‐phase (RP) stationary phases under pressure‐driven (viz. high performance liquid chromatography, HPLC) and electro‐driven (viz. capillary electrochromatography, CEC) conditions. Thermodynamic constants such as standard enthalpy, entropy, and Gibbs free energy were compared for both separation modes. Differences and/or similarities observed were used as a tool in gaining a better understanding of the separation mechanism in CEC compared to HPLC. In addition, effects of temperature on the magnitude of electroosmotic flow (EOF) are explained in detail.