Premium
Separation of conjugated linoleic acid isomers and parinaric fatty acid isomers by capillary electrophoresis
Author(s) -
Öhman Marcus,
Wan Hong,
Hamberg Mats,
Blomberg Lars G.
Publication year - 2002
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/1615-9314(20020601)25:8<499::aid-jssc499>3.0.co;2-x
Subject(s) - conjugated linoleic acid , chemistry , capillary electrophoresis , structural isomer , linoleic acid , chromatography , conjugated system , pulmonary surfactant , fatty acid , micelle , cis–trans isomerism , organic chemistry , biochemistry , polymer , aqueous solution
Separation of two types of fatty acid isomers, parinaric acid and conjugated linoleic acid, by capillary electrophoresis has been investigated. The performance of a number of different buffer systems was investigated. The use of SDS micelles combined with an organic modifier made it possible to dissolve and separate parinaric acid isomers (PA). For separation of conjugated linoleic acids (CLA) a buffer system containing a chiral surfactant (R)‐N ‐dodecoxycarbonylvaline ( (R)‐N ‐DOCV) and a mixture of two cyclodextrins, heptakis(2,3‐dimethyl‐6‐sulfo)‐β‐CD (HDMS‐β‐CD) and β‐CD, and organic modifier were utilized.