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Modeling and optimization for separation of ionic solutes in pressurized flow capillary electrochromatography
Author(s) -
Ye Mingliang,
Zou Hanfa,
Wu Renan,
Fu Hongqing,
Lei Zhengdeng
Publication year - 2002
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/1615-9314(20020501)25:7<416::aid-jssc416>3.0.co;2-7
Subject(s) - capillary electrochromatography , electrochromatography , chemistry , ionic strength , chromatography , capillary action , analytical chemistry (journal) , capillary electrophoresis , flow (mathematics) , electro osmosis , selectivity , correlation coefficient , streaming current , electrophoresis , materials science , mechanics , aqueous solution , membrane , composite material , biochemistry , statistics , mathematics , catalysis , physics
By manipulation of applied pressure or voltage, pressurized flow capillary electrochromatography (P‐CEC) permits unique control of selectivity for ionic solutes. A simple mathematical model has been developed to describe the quantitative relationship between the electrochromatographic retention factor ( k *) of charged solutes and the applied voltage and pressure. The validity of the model was verified experimentally with hydrophilic interaction mode CEC (HI‐CEC). On the basis of the model developed, it was found that the value of k * could be predicted accurately using only a limited number of data points from the initial experiments at different voltages or pressures. Correlation between the experimentally measured and calculated k * was excellent, with a correlation coefficient greater than 0.999. Optimization for the separation of peptides by P‐CEC was also performed successfully on the basis of the proposed model.