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Use of column temperature to optimize carotenoid isomer separation by C 30 high performance liquid chromatography
Author(s) -
Böhm Volker
Publication year - 2001
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/1615-9314(20011201)24:12<955::aid-jssc955>3.0.co;2-b
Subject(s) - chromatography , chemistry , xanthophyll , elution , lycopene , carotenoid , methanol , high performance liquid chromatography , resolution (logic) , column chromatography , analytical chemistry (journal) , organic chemistry , biochemistry , artificial intelligence , computer science
Five xanthophylls and six carotenes were eluted from a YMC C 30 column with a binary mobile phase gradient consisting of methyl tert ‐Butyl ether and methanol. The effect of column temperature on the separation was tested between 15°C and 25°C. A mixture of prominent lycopene isomers was additionally analyzed to optimize their separation in relation to the main xanthophylls and carotenes. At column temperatures below 20°C (15 Z )‐β‐carotene co‐eluted with echinenone, a frequently used internal standard. For most carotenoids an optimal separation was achieved at 23 ± 1°C within one hour. In contrast, some ( Z )‐isomers of lycopene showed a better resolution at higher column temperatures. While these isomers were separated at 30°C and at 35°C, another ( Z )‐isomer co‐eluted with ( E )‐lycopene at 35°C. Thus, column temperature is a noteworthy parameter in high performance liquid chromatography of carotenoids.