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The influence of column temperature on selectivity in reversed‐phase liquid chromatography for shape‐constrained solutes
Author(s) -
Sander Lane C.,
Wise Stephen A.
Publication year - 2001
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/1615-9314(20011201)24:12<910::aid-jssc910>3.0.co;2-a
Subject(s) - chemistry , selectivity , phase (matter) , chromatography , reversed phase chromatography , stationary phase , alkyl , column chromatography , column (typography) , chromatography column , high performance liquid chromatography , analytical chemistry (journal) , organic chemistry , catalysis , structural engineering , connection (principal bundle) , engineering
Relationships between column temperature and retention behavior are reviewed for separations in reversed‐phase liquid chromatography on hydrocarbonaceous bonded phases. Changes in selectivity are observed to occur with changes in temperature for a wide variety of stationary phase types. The largest temperature effects are observed for long alkyl chain length stationary phases and liquid crystalline stationary phases. Changes in stationary phase conformational structure are characterized by spectroscopic approaches, and correlations are made with retention behavior. In general, improved separations of isomers are usually possible at reduced column temperature. Applications are presented for classes of compounds that exhibit well‐defined (constrained) molecular structure.