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Fast detection of added soybean proteins in cow's, goat's, and ewe's milk by perfusion reversed‐phase high‐performance liquid chromatography
Author(s) -
Espeja Esther,
García M. Concepción,
Marina M. Luisa
Publication year - 2001
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/1615-9314(20011101)24:10/11<856::aid-jssc856>3.0.co;2-u
Subject(s) - chromatography , chemistry , trifluoroacetic acid , soybean proteins , reversed phase chromatography , high performance liquid chromatography , food science , soy protein
In order to detect possible adulterations in milk due to addition of soybean proteins a a perfusion reversed‐phase chromatographic column has been used with a linear acetonitrile‐water‐0.1% trifluoroacetic acid binary gradient for the determination of soybean proteins in cow's, goat's, and ewe's milk spiked with such proteins. The method permitted the simultaneous separation of soybean and milk proteins in less than 2 minutes at a flow‐rate of 5 mL/min and a temperature of 60°C. The method also enabled the quantitative analysis of soybean proteins in spiked milk, detecting up to 30 μg of soybean proteins/mL of milk. The method was validated by evaluation of its linearity, precision, and robustness and the possible existence of matrix interferences was also investigated for some of the milks. Although the external standard calibration method enabled the quantitation of soybean proteins in most of the milks examined, there were some cases in which the standard addition method was required.