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The use of silica for liquid chromatographic/mass spectrometric analysis of basic analytes
Author(s) -
McKeown Alan P.,
Euerby Melvin R.,
Lomax Helen,
Johnson Christopher M.,
Ritchie Harald J.,
Woodruff Mark
Publication year - 2001
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/1615-9314(20011101)24:10/11<835::aid-jssc835>3.0.co;2-5
Subject(s) - chemistry , chromatography , analyte , aqueous solution , adsorption , volume (thermodynamics) , fraction (chemistry) , silica gel , analytical chemistry (journal) , organic chemistry , physics , quantum mechanics
Silica stationary phases of varying purity have been compared and evaluated for the LC separation of pharmaceutically relevant basic compounds of differing molecular weight, log P , and p K a using aqueous/organic mobile phases. The least pure silica gave the best separations. All phases showed a linear relationship between ln k and volume fraction of ACN (30–70%) or MeOH (15–35%). The investigations confirmed that all the unbonded phases examined possessed a hydrophobic/adsorption and ion‐exchange character. The use of silica with MS “friendly” mobile phases, and high volume fractions of ACN, has been shown to be applicable to LC/MS analysis of basic analytes.