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Chiral separation of promethazine by capillary electrophoresis with end‐column amperometric detection
Author(s) -
Wang Rongying,
Lu Xiaoning,
Wu Mingjia
Publication year - 2001
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/1615-9314(20010801)24:8<658::aid-jssc658>3.0.co;2-6
Subject(s) - capillary electrophoresis , chromatography , amperometry , promethazine , chemistry , capillary electrochromatography , separation (statistics) , electrochromatography , electrophoresis , electrode , medicine , anesthesia , electrochemistry , machine learning , computer science
Sensitive end‐column amperometric detection has been successfully coupled to capillary electrophoresis for chiral separation of promethazine, with a carbon fiber microdisk electrode as working electrode. Baseline separation and sensitive detection were achieved under optimum conditions: 0.030 M Na 2 HPO 4 and 0.015 M citric acid at pH = 2.50, 1.0 mM β‐CD, 10 kV separation voltage, and detection potential 1.10 V (vs Ag/AgCl). The numbers of theoretical plates were higher than 700 000, and the detection limit was 5×10 –8 M. On‐line treatment of the electrode has also been studied and discussed.