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Evaluation of a novel non‐porous membrane extraction probe to determine sulphonylureas in plasma with analysis by LC‐MS/MS
Author(s) -
Mullins Frank G. P.
Publication year - 2001
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/1615-9314(20010801)24:7<593::aid-jssc593>3.0.co;2-f
Subject(s) - gliclazide , tolbutamide , chromatography , chemistry , glibenclamide , chlorpropamide , permeation , volunteer , membrane , extraction (chemistry) , diabetes mellitus , medicine , biochemistry , agronomy , biology , endocrinology
A silicone rubber thin membrane extraction probe was developed and evaluated as a sample preparation device prior to LC‐MS/MS analysis. The permeation properties of the membrane were evaluated using the five selected sulphonylureas, tolbutamide, gliclazide, chlorpropamide, tolazide, and glibenclamide. The effects of pH, temperature, and immersion time were investigated. A method for the determination of gliclazide in plasma was validated over the concentration range of 0.10–9.85 μg/mL covering the anticipated in‐vivo concentrations. The applicability of the method was demonstrated by the determination of plasma concentrations of gliclazide in a human volunteer following an 80 mg oral dose of gliclazide with blood sampling out to 48 hours.