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Temperature‐programmed non‐aqueous electrochromatographic separation of retinyl esters
Author(s) -
Roed Line,
Lundanes Elsa,
Greibrokk Tyge
Publication year - 2001
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/1615-9314(20010601)24:6<435::aid-jssc435>3.0.co;2-5
Subject(s) - chemistry , retinyl palmitate , chromatography , aqueous solution , electrochromatography , high performance liquid chromatography , acetonitrile , methanol , capillary electrochromatography , stationary phase , organic chemistry , retinol , vitamin , biochemistry
An in‐house assembled electrochromatograph was used for temperature‐controlled non‐aqueous electrochromatography. Reversed‐phase separations of retinyl esters were performed on continuous bed columns. The continuous bed columns were prepared by sol‐gel bonding of C 30 material in 180 μm internal diameter fused silica capillaries. The mobile phase consisted of 2.5 mM lithium acetate in N , N ‐dimethylformamide‐acetonitrile‐methanol (2 + 7 + 1, v / v ). The use of temperature programming increased the resolution of earlier‐eluting compounds as well as improved the peak shape and decreased the retention time of later‐eluting compounds. Separations of retinyl esters (all‐ trans ‐retinyl acetate, palmitate, heptadecanoate, stearate, oleate, and linoleate) were completed in 12 minutes. The within‐day and between‐day variations of retention times of all‐ trans ‐retinyl palmitate were <0.6% ( n = 6) relative standard deviation (RSD) and 2.3% ( n = 3) RSD, respectively. Further, the within‐day and between‐day variations of peak areas were < 2.7% ( n = 6) RSD, and 3.4% ( n = 3) RSD, respectively. The column examined was stable for more than two weeks of continuous injections of standard solutions and real samples. Lipid extracts of bearded seal ( Erignathus barbatus ) liver were analyzed.