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Comprehensive two‐dimensional capillary LC and CE for resolution of neutral components in traditional Chinese medicines
Author(s) -
Zhang Xiangmin,
Hu HuaLing,
Xu Shaoying,
Yang Xiuhan,
Zhang Jie
Publication year - 2001
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/1615-9314(20010501)24:5<385::aid-jssc385>3.0.co;2-u
Subject(s) - micellar electrokinetic chromatography , chromatography , electrokinetic phenomena , chemistry , capillary action , resolution (logic) , capillary electrophoresis , stacking , analytical chemistry (journal) , materials science , artificial intelligence , computer science , composite material , organic chemistry
Comprehensive two‐dimensional separation by capillary liquid chromatography coupled with capillary micellar electrokinetic chromatography was investigated. Fast micellar electrokinetic chromatography was achieved by speeding up electroosmotic flow and improving solute mass transfer in micellar pseudo‐stationary phase. High efficiency separation was achieved by selecting operating conditions based on theoretical relationships and experimental data. A new gating interface from the first dimension to the second dimension based on effluent stacking‐injection was developed. The reproducibility of the interface for sample transfer efficiency, migration time, and peak height was studied. The overall system performance was verified in the separation of complex neutral components. Resolution of hundreds of compounds present in traditional Chinese medicines was demonstrated.