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Inherent possibilities of improving recovery and selectivity using a long solid phase trap in analytical supercritical fluid extraction
Author(s) -
Eskilsson Cecilia Sparr,
Turner Charlotta,
Esbjörnsson Annelie,
Mathiasson Lennart
Publication year - 2001
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/1615-9314(20010401)24:4<297::aid-jssc297>3.0.co;2-q
Subject(s) - chemistry , chromatography , fractionation , selectivity , supercritical fluid , trap (plumbing) , boiling point , analyte , supercritical fluid extraction , extraction (chemistry) , elution , fraction (chemistry) , supercritical fluid chromatography , ion trap , solid phase extraction , boiling , trapping , phase (matter) , analytical chemistry (journal) , sample preparation , gas chromatography , mass spectrometry , organic chemistry , ecology , environmental engineering , engineering , biology , catalysis
Possibilities of improving recovery and selectivity in supercritical fluid extraction systems based on solid phase collection by using a longer trap (5×200 mm) instead of a standard trap (5×55 mm) were investigated. Such a long trap may give higher sample capacity, and filled with a chromatographic trapping material it gives a column with higher separation efficiency than the standard trap. The system was tested on thermally labile substances such as fat‐soluble vitamins and aromatic amines. Quantitative recoveries of both vitamins and amines were obtained when using the long trap combined with modifier in the extracting fluid and trapping temperatures below the boiling point of the modifier conditions that usually cause breakthrough losses of analytes. Furthermore, the vitamins could be eluted from the long trap well separated from the lipid fraction, while this was not possible using the standard trap. The possibility of obtaining selectivity between substances of similar structures, such as aromatic amines, was investigated on different types of trapping material (ODS, NH 2 , and CN). Fairly good fractionation was achieved using CN. This opens up the possibility to decrease the total analysis time by eluting the samples in fractions.