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Simultaneous determination of nine water‐soluble vitamins in pharmaceutical preparations by high‐performance liquid chromatography with diode array detection
Author(s) -
Li HuaBin,
Chen Feng
Publication year - 2001
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/1615-9314(20010401)24:4<271::aid-jssc271>3.0.co;2-l
Subject(s) - chromatography , high performance liquid chromatography , chemistry , chromatography detector , detection limit , calibration curve , quantitative analysis (chemistry) , reversed phase chromatography , methanol , analytical chemistry (journal) , organic chemistry
A simple and rapid method for the simultaneous determination of nine water‐soluble vitamins was developed by high performance liquid chromatography with diode array detection. The water‐soluble vitamins were analyzed by HPLC on a μBondapak C 18 column (300×3.9 mm, 10 μm) with methanol‐0.1 mol/L KH 2 PO 4 buffer (pH 7.0) as mobile phase (1.5 mL/min) in gradient mode as follows: in the range 0–7.0 min, 10:90; and in the range 8.0–15.0 min, 40:60 ( v / v ). All nine water‐soluble vitamins were separated in a single HPLC run within less than 15 min. The linearity of the calibration graphs was compound‐dependent with correlation coefficients of 0.9986 to 0.9999 ( n = 6). The detection limits ranged from 0.02 μg/mL to 0.5 μg/mL. The method was successfully applied to the determination of vitamins in pharmaceutical preparations. The recoveries were from 95% to 102% and the relative standard deviations were in the range of 0.8% to 4.2%.