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Analysis of guaifenesin‐based cough syrups by micellar electrokinetic chromatography and GC‐MS
Author(s) -
Pomponio Romeo,
Gotti Roberto,
Hudaib Mohammad,
Andrisano Vincenza,
Cavrini Vanni
Publication year - 2001
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/1615-9314(20010401)24:4<258::aid-jssc258>3.0.co;2-1
Subject(s) - chemistry , chromatography , micellar electrokinetic chromatography , sodium dodecyl sulfate , active ingredient , analyte , pulmonary surfactant , capillary electrophoresis , bioinformatics , biochemistry , biology
Capillary electrophoretic methods, under MEKC conditions, were developed for the simultaneous rapid analysis of neutral (guaifenesin) and basic (salbutamol and dextromethorphan) active ingredients of commercially available cough syrups. The running buffer nature played a key role on the migration of the studied analytes: using 0.04 M sodium dodecyl sulfate (SDS) as surfactant, sodium tetraborate and 3‐(cyclohexylamino)‐2‐hydroxy‐1‐propane‐sulfonic acid sodium salt (CAPSO) were chosen as running buffer allowing the separation of active ingredients and excipients of different cough syrups. Qualitative and quantitative information on the composition of the analyzed formulations were simultaneously obtained for both active ingredients and excipients by the proposed MEKC methods; when syrups containing volatile UV‐non‐absorbing components (flavors) were analyzed a GC‐MS approach was chosen. The aqueous matrices of the formulations allowed direct sample injection in CE, while extraction procedures were applied in order to perform GC analyses. The obtained data support the suitability of MEKC and GC‐MS as effective, complementary techniques for the selective and sensitive quality control of cough syrups.