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Influence of batch‐to‐batch reproducibility of Luna C 18 (2) packing material, nature of column wall material, and column diameter on the liquid chromatographic analysis of basic analytes
Author(s) -
Vervoort Ruud J. M.,
Ruyter Eddy,
Debets Alexander J. J.,
Claessens Henk A.,
Cramers Carel A.,
de Jong Gerhardus J.
Publication year - 2001
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/1615-9314(20010301)24:3<167::aid-jssc167>3.0.co;2-9
Subject(s) - chromatography , reproducibility , column (typography) , chemistry , analyte , van deemter equation , column chromatography , analytical chemistry (journal) , materials science , high performance liquid chromatography , geometry , connection (principal bundle) , mathematics
This paper discusses aspects arising on transferring liquid chromatography (LC) methods developed on conventional size columns to micro LC, i. e. the influence of batch‐to‐batch reproducibility of packing material, the nature of the column wall material, and the column inner diameter. It was shown that the nature of the column hardware as well as batches of Luna C18(2) packing material did not influence the retention of basic compounds. The batches of packing material, however, significantly influenced the peak shape for some compounds. The nature of the mobile phase buffer also showed an effect on peak shape. The column wall material as well as the column inner diameter did not reveal effects on the peak shape. Different packing densities were found for micro and conventional LC columns, i. e. the micro LC columns were less densely packed than the conventional LC columns. The study demonstrated that for the analysis of basic analytes similar separations are obtained using micro and conventional LC columns provided that the columns are packed from the same batch of packing material.