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A Practical NMR‐Based High‐Throughput Assay for Screening Enantioselective Catalysts and Biocatalysts
Author(s) -
Reetz Manfred T.,
Eipper Andreas,
Tielmann Patrick,
Mynott Richard
Publication year - 2002
Publication title -
advanced synthesis and catalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.541
H-Index - 155
eISSN - 1615-4169
pISSN - 1615-4150
DOI - 10.1002/1615-4169(200210)344:9<1008::aid-adsc1008>3.0.co;2-t
Subject(s) - enantioselective synthesis , chemistry , diastereomer , enantiomer , reagent , catalysis , throughput , combinatorial chemistry , high throughput screening , organic chemistry , computer science , telecommunications , biochemistry , wireless
Two NMR‐based approaches for high‐throughput screening of enantioselective catalysts and biocatalysts are described. One version makes use of pseudo ‐enantiomers or pseudo‐meso ‐compounds based on 13 C‐labeling. A throughput of at least 1400 ee determinations per day is possible by using an appropriate flow‐through cell and an autosampler. The other approach is based on traditional diastereomer formation using a chiral reagent or complexing agent. The ee values are accurate to within ±2% and ±5% of the true values.