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Integrated Biocatalytic Synthesis on Gram Scale: The Highly Enantioselective Preparation of Chiral Oxiranes with Styrene Monooxygenase
Author(s) -
Schmid Andreas,
Hofstetter Karin,
Feiten HansJürgen,
Hollmann Frank,
Witholt Bernard
Publication year - 2001
Publication title -
advanced synthesis and catalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.541
H-Index - 155
eISSN - 1615-4169
pISSN - 1615-4150
DOI - 10.1002/1615-4169(200108)343:6/7<732::aid-adsc732>3.0.co;2-q
Subject(s) - enantiopure drug , enantioselective synthesis , chemistry , styrene , biocatalysis , gram , organic chemistry , catalysis , monooxygenase , emulsion , epoxide , combinatorial chemistry , enzyme , polymer , copolymer , reaction mechanism , bacteria , cytochrome p450 , biology , genetics
Enantiopure oxiranes have been prepared on a gram scale by recombinant E. coli JM101 (pSPZ10) containing styrene monooxygenase in an emulsion process including facile and efficient downstream processing. This biocatalyst was compared to the chemical catalysts introduced by Jacobsen et al. with respect to its performance for epoxidations of vinyl groups.