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Homogenous Enzymatic Synthesis Using a Thermo‐Responsive Water‐Soluble Polymer Support
Author(s) -
Huang Xuefei,
Witte Krista L.,
Bergbreiter David E.,
Wong ChiHuey
Publication year - 2001
Publication title -
advanced synthesis and catalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.541
H-Index - 155
eISSN - 1615-4169
pISSN - 1615-4150
DOI - 10.1002/1615-4169(200108)343:6/7<675::aid-adsc675>3.0.co;2-2
Subject(s) - chemistry , polymer , polyacrylamide , precipitation , enzyme , glycosylation , chemical engineering , enzyme catalysis , immobilized enzyme , reuse , organic chemistry , chromatography , combinatorial chemistry , polymer chemistry , biochemistry , engineering , ecology , physics , meteorology , biology
Several enzymes immobilized on thermo‐responsive polyacrylamide polymers are nearly as active as their soluble forms, and can be recovered for reuse after gentle heating and precipitation. Carbohydrates attached to these polymers have been used for enzymatic glycosylation, and the products have been isolated by thermal precipitation followed by release from the polymer, thus greatly simplifying product purification in water.