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Parkin is associated with actin filaments in neuronal and nonneural cells
Author(s) -
Huynh Duong P.,
Scoles Daniel R.,
Ho Trang H.,
Del Bigio Marc R.,
Pulst StefanM.
Publication year - 2000
Publication title -
annals of neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.764
H-Index - 296
eISSN - 1531-8249
pISSN - 0364-5134
DOI - 10.1002/1531-8249(200011)48:5<737::aid-ana7>3.0.co;2-d
Subject(s) - parkin , neurodegeneration , biology , microbiology and biotechnology , parkinsonism , ubiquitin , pathology , neuroscience , parkinson's disease , genetics , gene , medicine , disease
Inactivating mutations of the gene encoding parkin are responsible for autosomal recessive juvenile parkinsonism (AR‐JP). However, little information is known about the function and distribution of parkin. We generated antibodies to two different peptides of parkin. By Western blot analysis and immunohistochemistry, we found that parkin is a 50‐kd protein that is expressed in neuronal processes and cytoplasm of selected neurons in the basal ganglia, midbrain, cerebellum, and cerebral cortex. Unlike ubiquitin and α‐synuclein, parkin labeling was not found in Lewy bodies of four sporadic Parkinson disease brains. Parkin was colocalized with actin filaments but not with microtubules in COS1 kidney cells and nerve growth factor–induced PC12 neurons. These results point to the importance of the cytoskeleton and associated proteins in neurodegeneration. Ann Neurol 2000;48:737–744