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An enzyme‐linked immunosorbent assay to quantify 14‐3‐3 proteins in the cerebrospinal fluid of suspected Creutzfeldt‐Jakob disease patients
Author(s) -
Kenney K.,
Brechtel C.,
Takahashi H.,
Kurohara K.,
Anderson Pat,
Gibbs C. J.
Publication year - 2000
Publication title -
annals of neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.764
H-Index - 296
eISSN - 1531-8249
pISSN - 0364-5134
DOI - 10.1002/1531-8249(200009)48:3<395::aid-ana18>3.0.co;2-a
Subject(s) - cerebrospinal fluid , medicine , pathology , disease , enzyme , virology , biology , biochemistry
The detection of 14‐3‐3 protein by Western immunoblot is a sensitive and specific cerebrospinal fluid marker of Creutzfeldt‐Jakob disease (CJD). We developed a quantitative enzyme‐linked immunosorbent assay (ELISA) that reliably detects 14‐3‐3 in cerebrospinal fluid. In a prospective study of 147 cerebrospinal fluid samples, the mean 14‐3‐3 concentration among pathologically confirmed CJD patients (28.0 ± 20.6 ng/ml, n = 41) is significantly higher than the mean in the cerebrospinal fluid of those with other neurological disorders (3.1 ± 2.9 ng/ml, n = 84). At a cutoff value of 8.3 ng/ml, the ELISA has a sensitivity of 92.7% and a specificity of 97.6%. The 14‐3‐3 ELISA supports a diagnosis of CJD in patients who fulfill clinical criteria for possible CJD. Ann Neurol 2000;48:395–398