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Nitration of manganese superoxide dismutase in cerebrospinal fluids is a marker for peroxynitrite‐mediated oxidative stress in neurodegenerative diseases
Author(s) -
Aoyama Koji,
Matsubara Kazuo,
Fujikawa Yasunori,
Nagahiro Yukio,
Shimizu Keiko,
Umegae Nobuyuki,
Hayase Nobumasa,
Shiono Hiroshi,
Kobayashi Shotai
Publication year - 2000
Publication title -
annals of neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.764
H-Index - 296
eISSN - 1531-8249
pISSN - 0364-5134
DOI - 10.1002/1531-8249(200004)47:4<524::aid-ana19>3.0.co;2-5
Subject(s) - amyotrophic lateral sclerosis , superoxide dismutase , peroxynitrite , oxidative stress , cerebrospinal fluid , chemistry , nitrotyrosine , dismutase , tyrosine , biochemistry , pathology , superoxide , medicine , enzyme , disease , nitric oxide synthase
Peroxynitrite can nitrate tyrosine residues of proteins. We examined nitrotyrosine‐containing proteins in cerebrospinal fluid of 66 patients with neurogenic disease by immunoblot analysis. Nitrated tyrosine residue–containing protein was observed in the cerebrospinal fluid and was concluded to be manganese superoxide dismutase (Mn‐SOD). The nitrated Mn‐SOD level was strikingly elevated in amyotrophic lateral sclerosis patients and was slightly increased in Alzheimer's and Parkinson's disease patients, whereas an elevated Mn‐SOD level was observed only in progressive supranuclear palsy group. Ann Neurol 2000;47:524–527.