Premium
Adult‐derived neural precursors transplanted into multiple regions in the adult brain
Author(s) -
Herrera Daniel G.,
GarciaVerdugo Jose Manuel,
AlvarezBuylla Arturo
Publication year - 1999
Publication title -
annals of neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.764
H-Index - 296
eISSN - 1531-8249
pISSN - 0364-5134
DOI - 10.1002/1531-8249(199912)46:6<867::aid-ana9>3.0.co;2-z
Subject(s) - olfactory bulb , subventricular zone , neuroblast , rostral migratory stream , biology , striatum , neuroscience , transplantation , olfactory ensheathing glia , neural stem cell , central nervous system , anatomy , neurogenesis , microbiology and biotechnology , stem cell , dopamine , medicine
Neural stem cells persist in the adult brain subventricular zone (SVZ). These cells generate a large number of new neurons that migrate to the olfactory bulb, where they complete their differentiation. Here, we transplanted cells carrying β‐galactosidase under the control of neuron‐specific enolase promoter (NSE::LacZ) from the SVZ of adult mice into the striatum cortex and olfactory bulb, with or without an excitotoxin lesion. Between 2 and 8 weeks after transplantation, grafted cells were present in the recipient regions, but extensive migration and differentiation into mature neurons of grafted cells were only observed in the olfactory bulb. Clusters of graft‐derived neuroblasts forming chain‐like structures were observed within or close to the grated sites in the cortex and striatum; electron microscopy confirmed that graft‐derived cells in the olfactory bulb and a small number in the striatum were neurons. Surprisingly, most of the cells expressing NSE::LacZ outside the olfactory bulb were astrocytes. We conclude that primary precursors from the SVZ migrate and differentiate effectively only within the environment of the olfactory bulb. Only limited survival and differentiation were observed in other brain regions studied