Open AccessAssociation of the course of collagen‐induced arthritis with distinct patterns of cytokine and chemokine messenger RNA expression
Author(s) -
Thornton Sherry,
Duwel Laura E.,
Boivin Gregory P.,
Ma Yuhe,
Hirsch Raphael
Publication year - 1999
Publication title -
arthritis & rheumatism
Language(s) - English
Resource type - Journals
eISSN - 1529-0131
pISSN - 0004-3591
DOI - 10.1002/1529-0131(199906)42:6<1109::aid-anr7>3.0.co;2-7
Subject(s) - chemokine , cytokine , arthritis , tumor necrosis factor alpha , immunology , messenger rna , ccl5 , macrophage inflammatory protein , rheumatoid arthritis , medicine , inflammation , biology , t cell , immune system , biochemistry , il 2 receptor , gene
Objective To quantitate changes in cytokine and chemokine messenger RNA (mRNA) levels during the development and progression of collagen‐induced arthritis (CIA) in mice. Methods Mice with CIA were scored for arthritis and killed at weekly intervals. Cytokine and chemokine mRNA levels were determined by RNase protection assays of total paw RNA. Results Arthritic paws exhibited mRNA levels of interleukin‐1β (IL‐1β), IL‐2, macrophage inflammatory protein 2 (MIP‐2), IL‐6, IL‐1 receptor antagonist, RANTES, tumor necrosis factor α (TNFα), TNFβ, MIP‐1α, IL‐11, transforming growth factor β1 (TGFβ1), TGFβ2, and TGFβ3 that were increased above mRNA levels in paws of normal, unimmunized mice and that exhibited distinct temporal patterns of mRNA expression. Clinically uninvolved paws also exhibited an increase in mRNA levels of IL‐11, RANTES, TNFα, TNFβ, and MIP‐1α. Conclusion The observed differential temporal cytokine and chemokine mRNA expression patterns suggest that specific cytokines and chemokines have defined roles at various times during the course of autoimmune arthritis. Since most of these cytokines and chemokines are found in human rheumatoid arthritis (RA) synovium and synovial fluids, these findings may have relevance to RA.