Premium
Template‐directed ligation: From DNA towards different versatile templates
Author(s) -
Leitzel John C.,
Lynn David G.
Publication year - 2001
Publication title -
the chemical record
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.61
H-Index - 78
eISSN - 1528-0691
pISSN - 1527-8999
DOI - 10.1002/1528-0691(2001)1:1<53::aid-tcr8>3.0.co;2-n
Subject(s) - template , ligation , dna , computer science , computational biology , chemistry , biology , programming language , microbiology and biotechnology , biochemistry
A systematic approach evaluating template‐directed ligation reactions has now resulted in a simple outline for a two‐stage replication cycle. This cycle builds on an efficient method for reading the information encoded in DNA into an amplified translation product. It is further demonstrated that the translation product strand is capable of catalyzing the synthesis of the original DNA strand. We propose that this cycle represents just one of many possible solutions; other chemical ligation or polymerization reactions could be accommodated with different templates. In that context, a new template, derived by modest changes to the DNA backbone, has been developed and has been shown to hybridize under reaction conditions different than those accessible to DNA. Therefore, the conceptual groundwork has been laid for extending this approach to encoding and reading stored information in molecules other than the natural biopolymers at the densities found in biology. © 2000 John Wiley & Sons, Inc. and The Japan Chemical Journal Forum Chem Rec 1:53–62, 2001