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Developmental mosaicism may explain spontaneous reappearance of the Axin Fu mutation in mice
Author(s) -
Ruvinsky Anatoly,
Flood Warren D.,
Costantini Frank
Publication year - 2001
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/1526-968x(200102)29:2<49::aid-gene1004>3.0.co;2-2
Subject(s) - biology , genetics , allele , mutant , locus (genetics) , mutation , microbiology and biotechnology , germline mosaicism , gene
Summary: The Axin Fu mutation is caused by an IAP insertion. In a small percentage of mice, the Axin Fu mutation disappears and is not observed in the next generation. In these mice, [ Axin Fu ]/+, the IAP is absent and Axin Fu has reverted to the wild allele. Concomitantly with the loss of IAP, there is widespread reorganisation of numerous microsatellite loci across the surrounding region of chromosome. In rare cases, spontaneous reappearance of the mutation can be observed in progeny of [ Axin Fu ]/+ mice. It is revealed here that reappearance of Axin Fu was associated with restoration of the IAP insertion. In such mice, alleles of the surrounding microsatellite loci were identical to the alleles observed on chromosomes that carried Axin Fu . Developmental mosaicism can potentially explain spontaneous reappearance of the Axin Fu mutation. Mosaicism can also explain other observations including postnatal changes at the Axin locus, unusual segregation in progeny, and the appearance of [ Axin Fu ]/+ mice that were phenotypically mutant. genesis 29:49–54, 2001. © 2001 Wiley‐Liss, Inc.