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Mouse embryonic stem (ES) cell lines established from neuronal cell‐derived cloned blastocysts
Author(s) -
Kawase Eihachiro,
Yamazaki Yukiko,
Yagi Takeshi,
Yanagimachi Ryuzo,
Pedersen Roger A.
Publication year - 2000
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/1526-968x(200011/12)28:3/4<156::aid-gene100>3.0.co;2-t
Subject(s) - embryonic stem cell , reprogramming , biology , somatic cell , induced pluripotent stem cell , microbiology and biotechnology , kosr , embryoid body , clone (java method) , stem cell , cell culture , p19 cell , somatic cell nuclear transfer , cell , blastocyst , embryo , genetics , embryogenesis , gene
Summary: We have established mouse embryonic stem (ES) cell lines from blastocysts derived by transfer of nuclei of fetal neuronal cells. These neuronal cell‐derived embryonic cell lines had properties that characterize them as ES cells, including typical cell markers and alkaline phosphatase activity. Moreover, the cells had a normal karyotype and were pluripotent, as they were capable of differentiating into all three germ layers. Although they were derived from neuronal donor nuclei, the cells no longer expressed neuronal markers; however, they were capable of differentiating into cells with neuronal characteristics. These results suggest that the clone‐derived cells have fully acquired an ES cell character. Thus, ES cells can be derived from embryos resulting from nuclear transfer, which results in reprogramming of the genetic information and acquisition of pluripotency. ES cells established from somatic cell‐derived blastocysts could be useful not only as research tools for studying reprogramming but also as models for cell‐based transplantation therapy. genesis 28:156–163, 2000. © 2000 Wiley‐Liss, Inc.