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Widespread recombinase expression using FLPeR (Flipper) mice
Author(s) -
Farley Francis W.,
Soriano Philippe,
Steffen Leta S.,
Dymecki Susan M.
Publication year - 2000
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/1526-968x(200011/12)28:3/4<106::aid-gene30>3.0.co;2-t
Subject(s) - recombinase , cre recombinase , biology , strain (injury) , locus (genetics) , gene , phenotype , genetics , gene expression , microbiology and biotechnology , transgene , genetically modified mouse , recombination , anatomy
Summary: As conditional genetic strategies advance, the need for multiple site‐specific recombinase systems has emerged. To meet this need in part, we have targeted the constitutive ROSA26 locus to create a mouse strain with generalized expression of the e nhanced version of the site‐specific recombinase FLP (FLPe). This strain is designated FLPeR (“flipper”). Using this strain, extensive target gene recombination can be achieved in most tissue types, including cells of the developing germ line. FLPeR mice therefore serve two important functions: as a source of many different FLPe‐expressing primary cell lines and as a deleter strain. Moreover, because the FLPeR mouse is a 129‐derived strain, a 129 genetic background can be preserved when crossed to most ES cell‐derived mice. This enables conditional genetic alterations to be maintained on a standard background, a feature important for obtaining reproducible results and genetically defined controls. genesis 28:106–110, 2000. © 2000 Wiley‐Liss, Inc.