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Universal GFP reporter for the study of vascular development
Author(s) -
Motoike Toshiyuki,
Loughna Siobhan,
Perens Elliot,
Roman Beth L.,
Liao Wayne,
Chau Tommy C.,
Richardson Charlene D.,
Kawate Toshimitsu,
Kuno Junko,
Weinstein Brant M.,
Stainier Didier Y. R.,
Sato Thomas N.
Publication year - 2000
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/1526-968x(200010)28:2<75::aid-gene50>3.0.co;2-s
Subject(s) - green fluorescent protein , cell sorting , transgene , biology , microbiology and biotechnology , reporter gene , zebrafish , morphogenesis , live cell imaging , genetically modified mouse , endothelial stem cell , cell , gene , gene expression , genetics , in vitro
Summary: We report the generation and characterization of transgenic mouse and zebrafish expressing green fluorescent protein (GFP) specifically in vascular endothelial cells in a relatively uniform fashion. These reporter lines exhibit fluorescent vessels in developing embryos and throughout adulthood, allowing visualization of the general vascular patterns with single cell resolution. Furthermore, we show the ability to purify endothelial cells from whole embryos and adult organs by a single step fluorescence activated cell sorting. We expect that these transgenic reporters will be useful tools for imaging vascular morphogenesis, global gene expression profile analysis of endothelial cells, and high throughput screening for vascular mutations. genesis 28:75–81, 2000. © 2000 Wiley‐Liss, Inc.