Premium
A new positive/negative selectable marker, puΔtk , for use in embryonic stem cells
Author(s) -
Chen YouTzung,
Bradley Allan
Publication year - 2000
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/1526-968x(200009)28:1<31::aid-gene40>3.0.co;2-k
Subject(s) - puromycin , selectable marker , thymidine kinase , biology , transfection , microbiology and biotechnology , embryonic stem cell , herpes simplex virus , stem cell , negative selection , transgene , cell culture , virology , genetics , virus , gene , protein biosynthesis , genome
Summary: A novel positive/negative selection cassette, puΔtk , was generated. puΔtk is a bifunctional fusion protein between puromycin N‐acetyltransferase (Puro) and a truncated version of herpes simplex virus type 1 thymidine kinase (ΔTk). Murine embryonic stem (ES) cells transfected with puΔtk become resistant to puromycin and sensitive to 1‐(‐2‐deoxy‐2‐fluoro‐1‐β‐D‐arabino‐furanosyl)‐5‐iodouracil (FIAU). Unlike other HSV1 tk transgenes, puΔtk is readily transmitted through the male germ line. Thus puΔtk is a convenient positive/negative selectable marker that can be widely used in many ES cell applications. genesis 28:31–35, 2000. © 2000 Wiley‐Liss, Inc.