Premium
A subtractive gene expression screen suggests a role for vanin‐1 in testis development in mice
Author(s) -
Bowles Josephine,
Bullejos Monica,
Koopman Peter
Publication year - 2000
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/1526-968x(200007)27:3<124::aid-gene50>3.0.co;2-e
Subject(s) - testis determining factor , biology , suppression subtractive hybridization , gene , sertoli cell , gonad , genetics , cdna library , mesonephros , in situ hybridization , development of the gonads , sexual differentiation , gene expression , disorders of sex development , complementary dna , microbiology and biotechnology , y chromosome , spermatogenesis , embryonic stem cell , anatomy , endocrinology
Summary: The molecular pathways leading from indifferent mammalian gonad to either testis or ovary are not well understood. A number of genes, including the Y‐linked sex determining gene SRY , have been shown to play roles in sex determination or differentiation, but there are clearly many missing elements to be found. We used suppression‐subtractive hybridization to construct normalized cDNA libraries enriched for male‐specific or female‐specific transcripts in mouse fetal gonads. We describe the strategy used to efficiently screen these libraries for candidate sex‐determination and gonadogenesis genes. One gene arising from these screens is vanin‐1, which encodes a protein implicated in the induction of cell migration into the thymus. We find that vanin‐1 is expressed male‐specifically in Sertoli cells of the developing testis and may be involved in inducing cell migration from the adjacent mesonephros, a process known to be critical for testis development. This screening approach is likely to be applicable to the isolation and study of genes involved in a variety of developmental systems. genesis 27:124–135, 2000. © 2000 Wiley‐Liss, Inc.