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Determination of anti‐oxidant activity by electron spin resonance spectroscopy
Author(s) -
Tran Thuy T,
Cronin Mark T D,
Dearden John C,
Morris Harry
Publication year - 2000
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/1526-4998(200009)56:9<818::aid-ps219>3.0.co;2-4
Subject(s) - scopoletin , chemistry , radical , electron paramagnetic resonance , catechin , spectroscopy , photochemistry , caffeic acid , nuclear chemistry , antioxidant , organic chemistry , nuclear magnetic resonance , polyphenol , medicine , physics , alternative medicine , pathology , quantum mechanics
A method to assess anti‐oxidant activity quantitatively is presented. In this method, free radicals (OH·) were produced from the Fenton reaction. Electron spin resonance spectroscopy (ESR) was then used to determine the effectiveness of five anti‐oxidants to scavenge the free radicals. Anti‐oxidant activity was assessed as the percentage reduction of the ESR signal intensity relative to that of a control after 10 min. The order of the potency of the antioxidants at 4.8 m M was: caffeic acid > o ‐coumarin > 6,7‐dihydroxy‐4‐methylcoumarin > catechin > scopoletin. In addition, pro‐oxidant activity (a higher ESR signal intensity than that of the control) was observed for catechin and scopoletin at low concentrations (below 3.6 m M ). © 2000 Society of Chemical Industry