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Cyanobacterial toxins detected by Thamnotoxkit (a double blind experiment)
Author(s) -
Törökné Andrea Kozma,
László Erzsébet,
Chorus Ingrid,
Sivonen Kaarina,
Barbosa Francisco A. R.
Publication year - 2000
Publication title -
environmental toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.813
H-Index - 77
eISSN - 1522-7278
pISSN - 1520-4081
DOI - 10.1002/1522-7278(2000)15:5<549::aid-tox27>3.0.co;2-z
Subject(s) - bioassay , cyanobacteria , toxin , biology , toxicity , chromatography , ecotoxicology , environmental chemistry , microbiology and biotechnology , toxicology , chemistry , ecology , bacteria , genetics , organic chemistry
The Thamnotoxkit F™ was evaluated for detecting cyanobacterial toxins compared with the rat hepatocyte test and mouse test. This test kit is a 24‐h bioassay using larvae of the freshwater anostracan crustacean Thamnocephalus platyurus hatched from cysts. Thirteen freeze‐dried cyanobacterial samples from freshwaters of Hungary, Germany, and Brazil, and strains from the culture collection of the University of Helsinki were tested and analyzed for microcystins and anatoxin a by high performance liquid chromatography and in some cases, the enzyme‐linked immunosorbent assay test. The samples were examined by a double blind approach. The Thamnotoxkit test reacts to pure microcystins, neurotoxins, and crude extracts of cyanobacteria. It can be concluded that the Thamnotox test is an alternative simple, cost‐effective method that may replace the mouse bioassay used previously for determination of cyanobacterial toxicity. © 2000 John Wiley & Sons, Inc. Environ Toxicol 15: 549–553, 2000