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Chemiluminescence activity in whole blood phagocytes of dogs naturally infected with Leishmania infantum
Author(s) -
Vuotto Maria Luisa,
De Luna Rocco,
Ielpo Maria Teresa Lucia,
De Sole Pasquale,
Moscatiello Vincenza,
Simeone Immacolata,
Gradoni Luigi,
Mancino Domenico
Publication year - 2000
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/1522-7243(200007/08)15:4<251::aid-bio589>3.0.co;2-b
Subject(s) - leishmania infantum , zymosan , leishmania , chemiluminescence , phagocyte , respiratory burst , opsonin , whole blood , microbiology and biotechnology , leishmaniasis , canine leishmaniasis , biology , immunology , parasite hosting , phagocytosis , visceral leishmaniasis , chemistry , biochemistry , in vitro , organic chemistry , world wide web , computer science
Dogs are the domestic reservoir of Leishmania infantum , a vector‐borne intracellular protozoan agent of human visceral leishmaniasis. The role of polymorphonuclear leukocytes (PMNs) in the immune defence against this parasite has been poorly studied. We have investigated the function of peripheral blood PMNs in naive beagle dogs that have been naturally exposed to phlebotomine vectors in an area highly endemic for canine leishmaniasis, and found infected by Leishmania at the end of the transmission season. Whole blood phagocyte oxidative metabolism was assessed by a rapid method that determines a luminol‐amplified chemiluminescence (CL) emission. This was evaluated using either a soluble stimulant, phorbol mirystate acetate (PMA), or phagocytic stimuli, such as zymosan unopsonized (ZYM) or opsonized with autologous serum (OPZ). In blood samples taken 2 months after exposure to Leishmania transmission, data on CL emission revealed a significant decrease of reactive oxygen intermediates (ROI) production in the presence of both PMA and ZYM, compared with blood samples obtained from dogs before exposure. On the contrary, no variations in CL emission were detected in presence of OPZ. Our data indicate that immunological changes occur early in canine leishmaniasis and confirm that the role of PMNs and their products need to be clarified. Copyright © 2000 John Wiley & Sons, Ltd.