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Chemiluminescence and reactive oxygen scavenging activities of the hydrogen peroxide/gallic acid/lactoperoxidase system
Author(s) -
Yoshiki Yumiko,
Okubo Kazuyoshi,
Akiyama Yoshinobu,
Sato Kaoru,
Kawanari Masami
Publication year - 2000
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/1522-7243(200005/06)15:3<183::aid-bio584>3.0.co;2-v
Subject(s) - gallic acid , chemiluminescence , chemistry , lactoperoxidase , hydrogen peroxide , photochemistry , nuclear chemistry , inorganic chemistry , peroxidase , organic chemistry , antioxidant , enzyme
Photon emission in the hydrogen peroxide/gallic acid/lactoperoxidase system was studied using visible region chemiluminescence. The photon emission intensity showed a pH‐dependence curve with a maximum at pH 4.5–5.0. The spectral analysis showed to be at 510 nm and its energy was 61.0 kcal/mol at either pH 4.5 or pH 7.0. In addition, near‐infrared spectral analysis at 1270 nm suggested that this photon emission from the hydrogen peroxide/gallic acid/lactoperoxidase system was produced without generation of 1 O 2 . The gallic acid/lactoperoxidase system, based on the chemiluminescence system, has superoxide‐, hydroxyl radical‐scavenging activities and antioxidative activity. These results are strong evidence that the gallic acid/lactoperoxidase system is one of the reactive oxygen scavenging systems. © 2000 John Wiley & Sons, Ltd.