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Effect of aqueous extract of cigarette smoke on peripheral blood polymorphonuclear leukocytes chemiluminescence
Author(s) -
Zappacosta Bruno,
Persichilli Silvia,
Giardina Bruno,
De Sole Pasquale
Publication year - 2000
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/1522-7243(200005/06)15:3<165::aid-bio582>3.0.co;2-x
Subject(s) - luminol , chemiluminescence , chemistry , horseradish peroxidase , cigarette smoke , smoke , lucigenin , aqueous solution , peroxidase , granulocyte , biochemistry , immunology , chromatography , enzyme , medicine , toxicology , superoxide , organic chemistry , biology
Cigarette smoke induces a vast cohort of deleterious effects on biological structures. In the present paper, the effect of aqueous extract of cigarette smoke on the activity of polymorphonuclear leukocytes was studied. Although the aqueous extract of cigarette smoke inhibits the luminol oxidation catalysed by horseradish peroxidase, it strongly interacts with polymorphonuclear leukocytes and inhibits their phorbol‐induced chemiluminescence in the presence of either luminol or lucigenin. The results indicate that at least some of the components of the aqueous extract of cigarette smoke may strongly interfere with polymorphonuclear cells, contributing to the deleterious effects of smoke products. © 2000 John Wiley & Sons, Ltd.