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Integration of a membrane‐based desalting step in a microfabricated disposable polymer injector for mass spectrometric protein analysis
Author(s) -
Lion Niels,
Gobry Véronique,
Jensen Henrik,
Rossier Joël S.,
Girault Hubert
Publication year - 2002
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200210)23:20<3583::aid-elps3583>3.0.co;2-n
Subject(s) - injector , elution , chromatography , polymer , chemistry , membrane , adsorption , mass spectrometry , analytical chemistry (journal) , materials science , organic chemistry , mechanical engineering , biochemistry , engineering
A simple desalting procedure for the coupling of a polymer microchip injector to mass spectrometry is proposed. The overall process is based on the adsorption of proteins on a poly(vinylidene difluoride) (PVDF) membrane, which are then directly eluted in the spraying solution. This microchip‐based approach has been successfully applied to small drugs, peptides and proteins originally diluted in phosphate‐buffered saline (PBS). Moreover, when eluting the retained proteins in small volumes, a preconcentration is obtained. The combination of single‐use, mass‐produceable, low‐sample‐consumption, easy‐to‐automate, miniaturized polymer injectors with easy‐to‐handle solution‐exchange membranes makes this system particularly amenable to screening applications.