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Performance of an in‐plane detection cell with integrated waveguides for UV/Vis absorbance measurements on microfluidic separation devices
Author(s) -
Petersen Nickolaj J.,
Mogensen Klaus B.,
Kutter Jörg P.
Publication year - 2002
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200210)23:20<3528::aid-elps3528>3.0.co;2-5
Subject(s) - capillary electrophoresis , capillary action , absorbance , microfluidics , materials science , analytical chemistry (journal) , sensitivity (control systems) , linear range , path length , optical path length , detection limit , optics , chemistry , chromatography , nanotechnology , physics , electronic engineering , engineering , composite material
A microfluidic device with integrated waveguides and a long path length detection cell for UV/Vis absorbance detection is presented. The 750 νm U‐cell detection geometry was evaluated in terms of its optical performance as well as its influence on efficiency for electrophoretic separations in the microdevice. Stray light was found to have a strong effect on both, the sensitivity of the detection and the available linear range. The long path length U‐cell showed a 9 times higher sensitivity when compared to a conventional capillary electrophoresis (CE) system with a 75 νm inner diameter (ID) capillary, and a 22 times higher sensitivity than with a 50 νm ID capillary. The linear range was comparable to that achieved in a 75 νm ID capillary and more than twice as large as in a 50 νm ID capillary. The use of the 750 νm U‐cell did not contribute significantly to band broadening; however, a clear quantification was made difficult by the convolution of several other band broadening sources.