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Optimization of the high‐frequency contactless conductivity detector for capillary electrophoresis
Author(s) -
Gaš Bohuslav,
Zuska Jiří,
Coufal Pavel,
van de Goor Tom
Publication year - 2002
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200210)23:20<3520::aid-elps3520>3.0.co;2-g
Subject(s) - capillary electrophoresis , detector , thermal conductivity detector , conductivity , chromatography , capillary action , electrophoresis , analytical chemistry (journal) , materials science , chemistry , physics , optics , composite material
Two constructions of the high‐frequency contactless conductivity detector that are fitted to the specific demands of capillary zone electrophoresis are described. The axial arrangement of the electrodes of the conductivity cell with two cylindrical electrodes placed around the outer wall of the capillary column is used. We propose an equivalent electrical model of the axial contactless conductivity cell, which explains the features of its behavior including overshooting phenomena. We give the computer numerical solution of the model enabling simulation of real experimental runs. The role of many parameters can be evaluated in this way, such as the dimension of the separation channel, dimension of the electrodes, length of the gap between electrodes, influence of the shielding, etc. The conception of model allows its use for the optimization of the construction of the conductivity cell, either in the cylindrical format or in the microchip format. The ability of the high‐frequency contactless conductivity detector is demonstrated on separation of inorganic ions.