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Estimation of isoelectric points of human plasma proteins employing capillary isoelectric focusing and peptide isoelectric point markers
Author(s) -
Jin Ya,
Luo Guoan,
Oka Tomoko,
Manabe Takashi
Publication year - 2002
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200210)23:19<3385::aid-elps3385>3.0.co;2-h
Subject(s) - isoelectric focusing , isoelectric point , pi , chromatography , chemistry , peptide , myeloma protein , human plasma , antibody , microbiology and biotechnology , biochemistry , biology , enzyme , immunology
Synthetic UV‐detectable peptide p I markers were used to estimate isoelectric point (p I ) values of proteins separated by capillary isoelectric focusing (CIEF) followed by cathodic mobilization in the absence of denaturing agents. The p I calculation and quantitative analysis of purified proteins showed the feasibility of these peptides as p I markers and internal standards in CIEF separation of proteins. Estimation of p I values of major proteins in human plasma was performed using the peptide p I markers, and the values were compared with those previously obtained by gel isoelectric focusing (IEF). Sera of immunoglobulin G (IgG) myeloma patients, which showed characteristic peaks of myeloma IgG in their CIEF patterns, were also subjected to the analysis and the p I values of the myeloma proteins have been estimated.